PBS Faculty Office: 4174

• BA 1976, Knox College
• PhD 1980, University of Wisconsin-Madison
• Postdoctoral Trainee, National Jewish Hospital, Denver CO 1980-1983.
• Professor in Microbiology

Immunoregulation of antibacterial resistance; food safety; immunotoxicology; bone marrow cells; cytokines; listeriosis; pasteurellosis; Haemophilus somnus; endothelial cells; Johne's disease; blastomycosis. The primary interest of our laboratory is studying mechanisms of resistance to bacterial infection. Under this broad heading major  projects include: 1) Host, bacterial and environmental factors that influence the pathogenesis of gastrointestinal listeriosis; 2) Immunotoxicology – adverse effects of polycyclic aromatic hydrocarbons on bone marrow cells and hematopoiesis; 3) Bovine respiratory disease - focus on the effects of Mannheimia haemolytica and its leukotoxin on the activities of bovine leukocytes and lung cells; 4) Haemophilus somnus – adverse effects on bovine endothelial cells as it relates to vasculitis; 5) Paratuberculosis – invasion and intracellular growth of M. paratuberculosis in bovine macrophages.

Teach Veterinary Bacteriology and Mycology (PBS 517); supervise graduate students in immunology, microbiology and toxicology; Director  of NIH Training Grant in Comparative Biomedical Sciences (provides research training to graduate veterinarians); PI of Summer Undergraduate Research Program in Molecular and Environmental Toxicology; Supervise Immunology Journal Club; Interim Director, UW  Food Research Institute 

Recent and Current Graduate Students

Recent and Current Postdoctoral Trainees

Atapattu, D. and C.J. Czuprynski. 2005. Mannheimia haemolytica leukotoxin induces apoptosis of bovine lymphoblastoid cells (BL-3) via a caspase-9 dependent mitochondrial pathway. Infect. Immun. 73:5504-5513.

Sylte, M.J., C. J. Kuckleburg, L. B. Corbeil, T. J. Inzana, P. J. Bertics, and C. J. Czuprynski. 2005. Stimulation of P2X₇ enhances Haemophilus somnus­ lipooligosaccharide-mediated apoptosis of endothelial cells. J. Leuk. Biol.  77:958-965.

Sylte, M.J.  C. J. Kuckleburg, F.P. Leite, T.J. Inzana and C.J. Czuprynski. 2005.  Interleukin-1 beta diminishes Haemophilus somnus­ lipooligosaccharide-mediated apoptosis of endothelial cells. Microb. Pathogen. 39:121-130.

Faith, N., G. Uhlich, J. Luchansky, B. Neudeck, and C.J. Czuprynski. 2005. A prfA transposon mutant of Listeria monocytogenes F2365 (serotype 4b) is able to survive in the gastrointestinal tract but not cause systemic infection of the spleen and liver of intragastrically inoculated mice. Infect. Immun. 73:7517-7524.

Galvan, N., C. J. CZUPRYNSKI, and C. Jefcoate. 2006. Benzo(a)pyrene and 7,12-dimethylbez(a)anthracene differentially affect bone marrow cells of the lymphoid and myeloid lineages. Toxicol. Appl. Pharmacol. 213:105-116

Sylte MJ, Kuckleburg CJ, Leite FP, Inzana TJ, Czuprynski CJ. 2006. Tumor necrosis factor-alpha enhances Haemophilus somnus lipooligosaccharide-induced apoptosis of bovine endothelial cells. Vet.  Immunol. Immunopathol. 110:303-309.

McClenahan^**, D. R. Kreuger, M. Kehrli, C. Thomas and C.J. CZUPRYNSKI. 2006. In situ hybridization analysis of interleukin-8 expression in bovine mammary gland tissue sections from calves with experimental Escherichia coli mastitis.  Comp. Immunol. Microbiol. Infect. Dis. 29:127-137

Behling-Kelly, E., H. Vonderheid, K.S. Kim, L. B. Corbeil, and C.J. CZUPRYNSKI. 2006. Roles of cellular activation and sulfated glycans in Haemophilus somnus adherence to bovine brain microvascular endothelial cells. Infect. Immun. 74:5311-5318

Woo SR, Sotos J, Hart AP, Barletta RG, Czuprynski CJ. 2006. Bovine monocytes and a macrophage cell line differ in their ability to phagocytose and support the intracellular survival of Mycobacterium avium subsp. paratuberculosis. Vet. Immunol. Immunopathol. 110:109-120.

Kuckleburg, C. J., S. F. Elswaifi, T. J. Inzana, and C.J. CZUPRYNSKI. 2007. Expression of phosphorylcholine by Histophilus somni induces bovine platelet aggregation. Infect. Immun.75:1045-1049.

Faith, N., S. Kathariou, Y. Cheng, N. Promadej, B. L. Neudeck, Q. Zhang, J. Luchansky, and C. Czuprynski. In revision. The role of Listeria monocytogenes serotype 4b gtcA in  gastrointestinal listeriosis in A/J mice. Foodborne Pathogen. Dis

Atapattu,. D., and C J. CZUPRYNSKI. 2007. Mannheimia haemolytica leukotoxin binds to lipid rafts in bovine lymphoblastoid cells (BL-3) and is internalized in a dynamin-2 and clathrin-dependent manner. Infect. Immun. 75:4719-4725

 Woo , S.R., J. A Heintz, R. Albrecht , R. G Barletta, and C. J. CZUPRYNSKI. 2007.  Life and death in bovine monocytes: the fate of Mycobacterium avium subsp. paratuberculosis. Microb. Pathogen. 43:106-113.

Peterson,  L.D.,  Faith, N.G., and C.J. CZUPRYNSKI. 2007. Resistance of  Listeria monocytogenes strain F2365 to synthetic gastric fluid is greater  following growth on ready-to-eat deli turkey meat than in brain heart  infusion broth. J. Food Protection. 70:2589-2595

Shi, L., N. G. Faith and C.J. CZUPRYNSKI. 2007. The aryl hydrocarbon receptor is required for optimal innate immunity to Listeria monocytogenes infection in mice. J. Immunol. 179:6952-6962.

Behling-Kelly, E, D. McClenahan, K.S. Kim, and C.J. Czuprynski. 2007. Viable Haemophilus somnus induce myosin light chain kinase-dependent decrease in brain endothelial cell monolayer resistance. Infect. Immun. 75:4572-4581.

Behling-Kelly, E., and C.J. Czuprynski. 2007. Endothelial cells as active participants in veterinary infections and inflammatory disorders. Anim. Health Res. Rev. 8:47-58.

McClenahan, D., K. Hellenbrand, D. Atapattu, N. Aulik, D. Carlton, A. Kapur, and C.J. Czuprynski. 2008. Effects of lipopolysaccharide and Mannheimia haemolytica leukotoxin on bovine lung microvascular endothelial cells and alveolar epithelial cells. Clin. Vaccine Immunol. 15:338-347.

Berryman, L. and C.J. CZUPRYNSKI. 2008. Flavonoid treatment of murine macrophages does not alter ingestion or intracellular survival of Listeria monocytogenes. Immunopharmacol.  Immunotoxicol. 30:217-226.

L.D. Peterson, Faith, N.G., and C.J. CZUPRYNSKI. 2008. Growth of Listeria monocytogenes strain F2365 on sliced, or in emulsified, ready-to-eat turkey meat does not enhance gastrointestinal listeriosis in intragastrically inoculated A/J mice. Int. J Food Microbiol. 126:112-115.

Kuckleburg, C., R. Tiwari, and C.J. CZUPRYNSKI. 2008. Endothelial cell apoptosis induced by bacteria-activated platelets requires caspase-8 and -9 and generation of reactive oxygen species. Thrombosis and Haemostasis 99:363-372

Woo, S. and C.J. CZUPRYNSKI. 2008. Tactics of Mycobacterium avium subsp. paratuberculosis for intracellular survival in mononuclear phagocytes. J Vet Sci. 9:1-8.

Neudeck, B., N. Faith, and C. Czuprynski. 2008. The poloxamer P85 is protective against Listeria monocytogenes invasion. Foodborne Pathogen. Dis. (in press).